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Laboratory Safety Manual and Chemical Hygiene Plan

LABORATORY SAFETY MANUAL AND CHEMICAL HYGIENE PLAN

TABLE OF CONTENTS 

 

Chapter 1.0 - Introduction

  • 1.1 Chemical Hygiene Plan Accessibility
  • 1.2 Laboratory Safety Responsibilities
    • 1.2.1 Environmental Health and Safety
    • 1.2.2 Chemical Hygiene Officer
    • 1.2.3 Deans, Directors, and Department Chairpersons
    • 1.2.4 Principal Investigators, Faculty, and Laboratory Supervisors
    • 1.2.5 Laboratory Employees
    • 1.2.6 Building Coordinators
    • 1.2.7 Department Safety Representatives 

 

Chapter 2.0 - Engineering Controls

  • 2.1 Chemical Fume Hoods
    • 2.1.1 Perchloric Acid Use
    • 2.1.2 Fume Hood Inspection and Testing Program
    • 2.1.3 Installation of New Fume Hoods
    • 2.1.4 Removal of Existing Fume Hoods
    • 2.1.5 Maintenance Procedures For Fume Hoods
  • 2.2 Other Capture Devices
  • 2.3 Glove Boxes
  • 2.4 Water Protection in Labs

 

Chapter 3.0 - Personal Protective Equipment

  • 3.1 Laboratory Responsibilities for Personal Protective Equipment
  • 3.2 Training for Personal Protective Equipment
  • 3.3 Eye Protection
    • 3.3.1 Eye Protection Selection
  • 3.4 Hand Protection
    • 3.4.1 Selecting the Proper Gloves
    • 3.4.2 Double Gloving
    • 3.4.3 Types of Gloves
  • 3.5 Protective Clothing
  • 3.6 Respirators
    • 3.6.1 Respiratory Protection Program
  • 3.7 Hearing Protection
  • 3.8 Foot Protection

 

  • 4.1 Standard Operating Procedures
  • 4.2 Form 10
  • 4.3 Procedural Controls
  • 4.4 Housekeeping
  • 4.5 Personal Hygiene
  • 4.6 Eating, Drinking, and Applying Cosmetics in the Lab
  • 4.7 Working Alone
  • 4.8 Phones in Labs
  • 4.9 Unattended Operations
  • 4.10 Access to Laboratories
    • 4.10.1 Visitors and Children in Labs
    • 4.10.2 Volunteers in Labs
    • 4.10.3 Visiting Scientists and Other Similar Users
    • 4.10.4 Pets in Labs
  • 4.11 Chemical Purchasing
  • 4.12 Ordering New Equipment
  • 4.13 Work Orders and Ticket Requests
  • 4.14 Changes in Lab Occupancy
  • 4.15 Laboratory Design and Construction
  • 4.16 Ventilation Rates
    • 4.16.1 Room Air Pressure in Labs
  • 4.17 Energy Conservation in Laboratories
  • 4.18 Green Labs
  • 4.19 Research Area Inspections
    • 4.19.1 Self Inspections
    • 4.19.2 Inspections by Regulatory Agencies
  • 4.20 Research Area Space Registration Using HASP
  • 4.21 Laboratory Security
    • 4.21.1 Security Guidelines

 

  • 5.1 Cornell Emergency Plan
    • 5.1.1 Unit Emergency Planning
    • 5.1.2 Evacuation Maps
  • 5.2 Emergency Evacuation Procedures
  • 5.3 Emergency Procedures
    • 5.3.1 Laboratory Emergency Shutdown Procedures
    • 5.3.2 Medical Emergency Procedures
    • 5.3.3 First Aid Kits
    • 5.3.4 Fire or Explosion Emergency Procedures
    • 5.3.5 Fire Extinguishers
    • 5.3.6 Power Outage Procedures
  • 5.4 Chemical Spill Procedures
    • 5.4.1 Incidental Spills
      • 5.4.1.1 Incidental Spill Cleanup Procedures
    • 5.4.2 Spill Absorbent Materials
    • 5.4.3 Spill Kits
    • 5.4.4 Major Spills
      • 5.4.4.1 Major Spill Cleanup Procedures
  • 5.5 Emergency Eyewash and Showers
    • 5.5.1 Testing and Inspection of Emergency Eyewash and Showers
    • 5.5.2 Installation for New Emergency Eyewash and Showers
    • 5.5.3 Maintenance Procedures For Emergency Eyewash and Showers
    • 5.5.4 Using Emergency Eyewash and Showers
  • 5.6 Injury/Illness Reporting
  • 5.7 Medical Consultations
    • 5.7.1 Information Provided to the Physician
    • 5.7.2 The Physician’s Written Opinion

 

  • 6.1 Training Options
  • 6.2 Cornell Learning Management System
  • 6.3 Laboratory Safety Certificate Program
  • 6.4 Research Administration Certification Program

 

Chapter 7.0 - Safe Chemical Use

  • 7.1 Minimize Exposure to Chemicals
  • 7.2 Understanding Chemical Hazards
    • 7.2.1 Chemical Hazard Information
  • 7.3 Material Safety Data Sheets
    • 7.3.1 SDSs and Newly Synthesized Chemicals
  • 7.4 Routes of Chemical Entry
    • 7.4.1 Inhalation
    • 7.4.2 Ingestion
    • 7.4.3 Injection
    • 7.4.4 Eye and Skin Absorption
  • 7.5 Chemical Exposure Limits
  • 7.6 Chemical Exposure Monitoring
  • 7.7 Toxicity
    • 7.7.1 Toxic Effects
    • 7.7.2 Evaluating Toxicity Data
  • 7.8 Chemical Labeling
    • 7.8.1 Non-Original Containers
    • 7.8.2 Labeling Requirements
  • 7.9 Chemical Storage
    • 7.9.1 General Storage Guidelines
  • 7.10 Transporting Chemicals
  • 7.11 Chemical Segregation
    • 7.11.1 EH&S Modified DOT Hazard Class System

 

  • 8.1 Explosives
  • 8.2 Flammable and Combustible Liquids
    • 8.2.1 Flammable Storage in Refrigerators/Freezers
    • 8.2.2 Flammable Storage Cabinets
  • 8.3 Flammable Solids
  • 8.4 Spontaneously Combustible
  • 8.5 Dangerous When Wet
  • 8.6 Oxidizers and Organic Peroxides
  • 8.7 Peroxide Forming Compounds
  • 8.8 Poisons
  • 8.9 Corrosives
    • 8.9.1 Hydrofluoric Acid
    • 8.9.2 Perchloric Acid

 

  • 9.1 Establishment of a Designated Area
  • 9.2 Safe Removal of Contaminated Materials and Waste
  • 9.3 Decontamination Procedures
  • 9.4 Guidelines for Working with Particularly Hazardous Substances
  • 9.5 Prior Approval
  • 9.6 Campus Prior Approval
  • 9.7 Select Carcinogens
  • 9.8 Reproductive Toxins
  • 9.9 Acute Toxins
     

 Chapter 10.0 - Hazardous Chemical Waste Disposal

  • 10.1 Hazardous Chemical Waste Container Requirements
  • 10.2 Hazardous Waste Pickup Procedures

 

  • 11.1 Regulated Hazardous Materials
  • 11.2 Hazardous Materials Transportation Requirements

 

Chapter 12.0 - Pesticides

  • 12.1 Pesticide Certification
    • 12.1.1 Exemptions from Pesticide Certification

 

  • 13.1 Institutional Biosafety Committee
    • 13.1.1 Recombinant DNA
    • 13.1.2 Infectious and Pathogenic Agents
    • 13.1.3 Select Biological Agents and Toxins
    • 13.1.4 Gene Therapy
  • 13.2 Animal Use
  • 13.3 Human Participants
  • 13.4 Shipping Biological Materials
    • 13.4.1 Permits for the Import and Export of Biological Materials
  • 13.5 Biological Safety Cabinets
    • 13.5.1 Biological Safety Cabinet Certifications
    • 13.5.2 BSC Work Practices and Procedures
    • 13.5.3 BSC Operational Procedures
    • 13.5.4 Use of Ultraviolet Lights in the BSC
    • 13.5.5 Types of Biological Safety Cabinets
  • 13.6 Biohazardous Waste (Regulated Medical Waste)
    • 13.6.1 Hypodermic Syringes and Needles

 

  • 14.1 Where Ionizing Radiation is Used
  • 14.2 Potential Hazards
    • 14.2.1 How to Protect Yourself
  • 14.3 Control of Ionizing Radiation
  • 14.4 Radioactive Waste Disposal

 

 
 
  • 16.1 Electrical Safety
    • 16.1.1 Common Electrical Hazards and Preventative Steps
    • 16.1.2 Safe Use of Electrophoresis Equipment
  • 16.2 Machine Guarding
    • 16.2.1 Machine Safety Responsibilities
    • 16.2.2 Common Machine Hazards
  • 16.3 Lighting
  • 16.4 Compressed Gases
    • 16.4.1 Handling Compressed Gas Cylinders
    • 16.4.2 Safe Storage of Compressed Gas Cylinders
    • 16.4.3 Operation of Compressed Gas Cylinders
    • 16.4.4 Return of Cylinders
    • 16.4.5 Hazards of Specific Gases
  • 16.5 Battery Charging
  • 16.6 Heat and Heating Devices
    • 16.6.1 Heat Stress
  • 16.7 Cold Traps
  • 16.8 Autoclaves
  • 16.9 Centrifuges
    • 16.9.1 Centrifuge Rotor Care
  • 16.10 Cryogenic Safety
    • 16.10.1 Cryogenic Safety Guidelines
    • 16.10.2 Cryogenic Chemical Specific Information
  • 16.11 Extractions and Distillations
  • 16.12 Glass Under Vacuum
  • 16.13 Glassware Washing
  • 16.14 General Equipment Set Up
    • 16.14.1 Glassware and Plasticware
    • 16.14.2 Preparation of Glass Tubing and Stoppers
    • 16.14.3 Insertion of Glass Tubes or Rods into Stoppers
    • 16.14.4 Assembling Apparatus
    • 16.14.5 Mercury Containing Equipment
  • 16.15 Ergonomics

 

 

Appendix A: Chemical Hygiene Plan

 

Appendix B: Contact List

 

Appendix C: Compiled PI Lab Safety Responsibilities

 

Appendix D: Standard Operating Procedure Examples

 

Appendix E: Lab Move Guide

 

Appendix F: Glove Selection For Specific Chemicals

 

Appendix G: EH&S Video Library

 

Appendix H: How To Understand MSDSs

 

Appendix I: Hazards Of Functional Groups

 

Appendix J: Peroxide Forming Chemicals

 

Appendix K: Incompatible Chemicals

 

Appendix L: EH&S Segregation Scheme

 

Appendix M: Sample Prior Approval Form

 

Appendix N: Laser Registration Form

 

Appendix O: EH&S Reference Library  

 

Appendix P: Phenol First Aid Guide

 

 
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